Flexible WorkFlow NGS Solutions

Don’t need full service sequencing? myGenomics provides workflow solutions customized to your requirements

myGenomics is happy to extend a helping hand in whatever step you need one. As a high throughput NGS lab, equipped with automated liquid handlers, myGenomics has the expertise and resources to provide you many workflow solutions with better quality, faster turnaround and more cost efficient way. Please feel free to contact us to discuss your project aim. We provide help in various molecular biology applications. Some of them include:

High Quality Nucleic Acid Extraction and Purification Service

  • DNA extraction from Blood, Saliva, tissue or FFPE sections
  • RNA extraction from blood, tissue or FFPE: PolyA RNA, miRNA, exosomal RNA
  • Quality control by Qubit, nanodrop and electrophoretic profile

NGS library preparation

  • High throughput volume handled through Agilent Bravo
  • Mate-pair libraries for larger fragment size inserts
  • QC by Q-PCR, Qubit, TapeStation Wide variety of sample types accepted: DNA, RNA, ChIP, Amplicons and more

MiSeq Flow cell Rental

  • You can send the sequencer ready libraries to be run on the MiSeq. Sequencing formats available:
  • PE100, PE150, PE250, PE300, SE50, SE150
  • Turnaround time: 5 business days (projects have been delivered in 3 days).
  • User assumes responsibility for the flow cell output for libraries not prepared by myGenomics.

HiSeq Lane Rental

  • You can send the sequencer ready libraries to be run on per lane basis on HiSeq flow cell.
  • PE100 is the default sequencing format. Other sequencing formats are available if minimum 4 lanes are requested and are run on HiSeq2500 or NextSeq500.
  • Turnaround time: 20-30 business days.
  • User assumes responsibility for the flow cell output for libraries not prepared by myGenomics.

NextSeq500 Flow Cell Rental

  • You can send the sequencer ready libraries to be run on NextSeq500 flow cell.
  • SE75, PE75, SE150, PE150
  • Mid output (130 million SE reads and 260 million PE reads) and
    High Output (400 million SE reads and 800 million PE reads)
  • Turnaround time: 10-15 business days.
  • User assumes responsibility for the flow cell output for libraries not prepared by myGenomics.

Real time Quantitative PCR

  • Agilent Mx30005P
  • High sensitive quantification and validation
  • TaqMan Genotyping and Expression Analysis
  • Pathogen Detection
  • Turnaround time: 2-3 business days.

PCR amplification and Sanger sequencing

  • In-silico primer design to find the minimum number of amplicons required to cover the desired target. Order oligos for synthesis.
  • PCR amplification and purification
  • Single end or paired end sequencing to cover the target with high quality sequencing reads
  • Data analysis and variant call.

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