Sequencing the coding regions or the whole cancer transcriptome can provide valuable information about gene expression changes in tumors. Cancer RNA-Seq enables detection of strand-specific information, an important component of gene regulation. Cancer transcriptome sequencing captures both coding and noncoding RNA and provides strand orientation for a complete view of expression dynamics.

Targeted RNA amplicon sequencing analyzes the expression of specific cancer genes. Predesigned cancer gene expression panels focus on relevant pathways, such as p53 and NFκB. Researchers can add custom content to broaden the scope of an experiment.

Genomic regions containing deleted or amplified miRNA loci have been linked to multiple forms of cancer. Small RNA-Seq enables the discovery and profiling of miRNAs and other small, noncoding RNAs present in the cancer transcriptome.

Formalin-fixed, paraffin-embedded (FFPE) tumor samples potentially contain valuable information about cancer pathogenesis and outcomes. Because FFPE samples generally contain partially degraded RNA, cancer RNA-Seq can pose challenges. Next-generation RNA-Seq methods enable researchers to access this important data, yielding high-quality results from degraded and FFPE-derived RNA.